spoIVFB [2017-12-04 14:14:12]

intramembrane metalloprotease, processing of pro-sigma-K to active SigK

Locus: BSU27970

Isoelectric point: 8.48

Molecular weight: 33.49 kDa

Protein length: 288 aa

Gene length: 864 bp

Function: processing of pro-sigma-K to active SigK

Product: intramembrane metalloprotease

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Proteolysis → Proteolysis during sporulation/ germination
• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,855,973 → 2,856,839

The protein

Catalyzed reaction/ biological activity

• processing of pro-sigma-K to active SigK PubMed

Protein family

• peptidase M50B family (according to Swiss-Prot)

Domains

• C-terminal cystathionine-beta-synthase (CBS) domain, this domain binds ATP PubMed

Effectors of protein activity

• ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level PubMed

Localization

• integral membrane protein PubMed
• mother cell membrane PubMed

Expression and Regulation

Operons

• Genes: spoIVFA-spoIVFB
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Additional information

  • the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

• Genes: spoIVFA-spoIVFB-rplU-prp-rpmA
  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Additional information

  • the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

Biological materials

Mutant

• BKE27970 (ΔspoIVFB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC
• BKK27970 (ΔspoIVFB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC

References

Reviews

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Original Publications

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